Solubility | H2O: soluble1mg/mL |
Appearance | lyophilized powder |
Color | white |
Storage Condition | 2-8°C |
In vitro study | Streptolysin O is a labile toxin, and, with time, activity is irreversibly lost. The rate of activity loss is slowed by incubation with 0.1 M 2-mercaptoethanol or 0.01 M ethylenediaminetetraacetic acid (EDTA). Streptolysin O, belongs to the group of thiol-activated toxins, is a secreted virulence factor of GAS that induces apoptosis in neutrophils and macrophages at later time points (~4 h). Streptolysin O induces eukaryotic cell lysis in a cholesterol-dependent manner, is highly upregulated in the GAS M1T1 clone during bloodstream dissemination. Streptolysin O is known to promote Group A Streptococcus (GAS) resistance to phagocytic clearance by neutrophils, a critical first element of host defense against invasive bacterial infection. |
UN IDs | UN 2811PIH 6.1 / PGI |
WGK Germany | 3 |
The liquid preparation is a light red or light yellow clear liquid; The freeze-dried preparation is a light yellow or light red loose mass, which is rapidly dissolved after adding the diluent.
This strain inoculated rabbits with sheep red blood cells as antigen, collected blood, separated serum, made by freeze-vacuum drying, or made into liquid preparation by adding glycerol. The same amount of physiological saline was added when the lyophilized preparation was used.
The liquid hemolysin is stored at 2~8 ℃, and is valid for 12 months. Lyophilized hemolysin stored below 8 ℃, valid for 60 months.
classification | according to the hemolytic ability of sheep red blood cells, it can be divided into: ① type a (a) hemolytic streptococcus, also known as streptococcus viridans, is a normal host bacteria of human respiratory tract and intestinal tract, with low pathogenicity. ② B (β) hemolytic streptococcus, also known as hemolytic streptococcus, has strong pathogenicity and can cause many diseases in humans or animals. ③ Streptococcus C, also known as non-hemolytic streptococcus, is generally non-pathogenic and often exists in dairy products and feces. Among them, the most studied is beta (β) hemolytic streptococcus. |
biological activity | Streptolysin o, group a streptococcal toxin, is an oxygen unstable prototype cholesterol binding bacterial exotoxin. Streptolysin O leads to cell lysis and cardiotoxicity. Streptolysin O is used for controllable permeability of cell membranes. Streptolysin O exists in two forms, the reductive active state and the oxidative reversible inactive state. |